Journal: Heliyon

Article Title: Urothelial bladder afferents selectively project to L6/S1 levels and are more peptidergic than those projecting to the T13/L1 levels in female rats

doi: 10.1016/j.heliyon.2023.e18495

Figure Lengend Snippet: Cell Count; Cell Diameters (μm±SEM) of bPANs.

Article Snippet: Tissues were then incubated overnight at 4°C in blocking buffer containing the following primary antibodies: polyclonal rabbit anti-CGRP primary antibody (1:2000; ImmunoStar cat. #24112) or polyclonal guinea pig anti-SP primary antibody (1:1000; Abcam cat. #10353).

Techniques: Cell Counting, Labeling, Staining

Journal: Heliyon

Article Title: Urothelial bladder afferents selectively project to L6/S1 levels and are more peptidergic than those projecting to the T13/L1 levels in female rats

doi: 10.1016/j.heliyon.2023.e18495

Figure Lengend Snippet: Representative examples of dorsal root ganglion neurons retrogradely labeled with Fast Blue and DiI, and immunohistochemically stained with antibodies against CGRP (Panel A) and SubP (Panel B). White arrows indicate DiI-labeled neurons. (For interpretation of the references to colour in this figure legend, the reader is referred to the Web version of this article.)

Article Snippet: Tissues were then incubated overnight at 4°C in blocking buffer containing the following primary antibodies: polyclonal rabbit anti-CGRP primary antibody (1:2000; ImmunoStar cat. #24112) or polyclonal guinea pig anti-SP primary antibody (1:1000; Abcam cat. #10353).

Techniques: Labeling, Staining

Journal: The Journal of Clinical Investigation

Article Title: Mechanisms and treatments of neuropathic itch in a mouse model of lymphoma

doi: 10.1172/JCI160807

Figure Lengend Snippet: ( A ) Double immunostaining for ATF3 and IB4 in cervical DRGs of naive and CTCL mice at day 60. Scale bar: 25 μm. ( B ) Double immunostaining of ATF3 and the neuronal markers NF200, P2X3, and CGRP in cervical DRGs from CTCL mice at day 60. Scale bar: 25 μm. ( C ) Percentage of ATF3 + , IB4 + , NF200 + , P2X3 + , and CGRP + neurons in the cervical DRG of CTCL mice at different time points. n = 3. Two-way ANOVA, F (12, 30) = 9.15, P < 0.0001. ( D ) Percentage of ATF3 colocalization with P2X3 + neurons in the cervical DRG of CTCL mice at different time points. n = 3. One-way ANOVA, F (3, 8) = 31.89, P < 0.0001. ( E ) Pie chart showing ATF3 colocalization with NF200 + , P2X3 + , and CGRP + neurons in the cervical DRG from a CTCL mouse on day 60. Data were collected from 3 animals. ( F ) Double immunostaining for CGRP and IB4 in the cervical spinal cord dorsal horn from CTCL mice at day 60. Arrowheads indicate the loss of IB4 + primary afferents in the IIi. Scale bar: 100 μm. Data are expressed as the mean ± SEM. One- or 2-way ANOVA with Bonferroni’s post hoc test, ** P < 0.01, *** P < 0.001, and **** P < 0.0001 versus the naive group.

Article Snippet: The primary antibodies included anti-CGRP antibody (goat, 1:400, Bio-Rad, catalog 1720-9007), anti-ATF3 antibody (mouse, 1:400, Santa Cruz Biotechnology, catalog sc-81189), anti-NF200 antibody (rabbit, 1:5,000, Abcam, catalog ab8135), anti-P2X3 antibody (mouse, 1:500, Neuromics, catalog Gp10108), anti–β–tubulin III antibody (mouse, 1:500, R&D Systems, catalog MAB1195), anti-CD68 antibody (rat, 1:500, BioLegend, catalog 137011), anti–Gr-1 antibody (rat, 1:500, BioLegend, catalog 127623), anti-CD11c antibody (Armenian hamster, 1:500, BD Pharmingen, catalog 550283), cleaved caspase 3 antibody (rabbit, 1:400, Cell Signaling Technology, catalog 9661), and anti–IB4–Alexa Fluor 488 conjugate (Invitrogen, Thermo Fisher Scientific, 1:1,000, catalog 121411).

Techniques: Double Immunostaining